Although THINC’s products may ultimately integrate multiple Telome Measures from multiple cell types, to date, most studies have utilized circulating white blood cells from blood and only average TL is measured. Average TL by qPCR (quantitative polymerase chain reaction) is by far the simplest and most scaleable assay for clinical applications.
Our current business strategy focuses primarily on THINC’s first product, the “Cawthon qPCR assay” for average TL . The foundational patent on this technology has been issued in the US and other countries, and THINC has the exclusive worldwide rights to it and related Cawthon patents.
Using our specific primers for telomeric DNA and assay protocols, a sample having longer telomeres will yield quantitatively more qPCR product than a sample with shorter telomeres. THINC uses multiple standards and normalization procedures to ensure precision and accuracy of the assay.
The ratio of the telomeric signal vs. the single copy gene signal reflects the average length of the telomeres per genomic DNA in the sample. THINC's founders have developed extensive and proprietary methods to tackle the sensitive nature of the qPCR telomere length assay to sample collection, storage, preparation, and assay conditions.